Cloned (Comment) | Organism |
---|---|
expressed using a tac promoter-driven N-terminal GST fusion protein | Synechococcus sp. |
into pASK-IBA45+ and transformed in Escherichia coli DH10B cells | Microchaete diplosiphon |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microchaete diplosiphon | Q6UR87 | - |
- |
Microchaete diplosiphon Fd33 | Q6UR87 | - |
- |
Synechococcus sp. | - |
- |
- |
Synechococcus sp. WH8020 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Microchaete diplosiphon |
by two affinity chromatography steps to 90% purity and a third gel filtration step | Synechococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15,16-dihydrobiliverdin + reduced ferredoxin | - |
Synechococcus sp. | (3Z)-phycoerythrobilin + oxidized ferredoxin | - |
r | |
15,16-dihydrobiliverdin + reduced ferredoxin | - |
Microchaete diplosiphon | (3Z)-phycoerythrobilin + oxidized ferredoxin | - |
r | |
15,16-dihydrobiliverdin + reduced ferredoxin | - |
Synechococcus sp. WH8020 | (3Z)-phycoerythrobilin + oxidized ferredoxin | - |
r | |
15,16-dihydrobiliverdin + reduced ferredoxin | - |
Microchaete diplosiphon Fd33 | (3Z)-phycoerythrobilin + oxidized ferredoxin | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PebB | - |
Synechococcus sp. |
PebB | - |
Microchaete diplosiphon |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Synechococcus sp. | |
Ferredoxin | - |
Microchaete diplosiphon | |
NADP+ | - |
Synechococcus sp. | |
NADP+ | - |
Microchaete diplosiphon |